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The proprietary Line of LIPO-PRO™ adipose processing systems Offer clean and gentle processes for creating High Density fat grafts of various volumes.  

The LIPO-PRO™ in-process filtration method micronizes fat during centrifugation and isolates high-density fat from low-density fat.*

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LP-30

Part #: 76091-01M

Process up to 30cc’s of lipoaspirate (up to 10cc final product)

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LP-150

Part #: 76091-02M

Process up to 150cc’s of lipoaspirate.

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LP-EXP

Part #: 76091-03M

The LIPO-PRO™ expansion pack provides tools to process lipoaspirate infranatant, allowing clinicians to acquire the High-Density Cellular Fraction (HDCF) with minimal manipulation.

*“Greater percentages of highest density fractions of lipoaspirate persist over time compared with lowest density fractions. A vasculogenic mechanism appears to contribute significantly, as highest density fractions contain more progenitor cells and increased concentrations of several vasculogenic mediators than lowest density fractions.”

Allen RJ Jr, “Grading lipoaspirate: is there an optimal density for fat grafting?” Plast Reconstr Surg. 2013 Jan;131(1):38-45.

 

Frequently Asked Questions

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What are the Indications for Use for the LIPO-PRO System?

The Ranfac LIPO-PRO adipose processing kit is used in medical procedures involving the harvesting and transferring of autologous adipose tissue. The LIPO-PRO kit is for concentrating adipose tissue harvested with a legally marketed lipoplasty system. The device is intended for use in the following surgical specialties when the concentration of harvested adipose is desired:
Neurosurgery, gastrointestinal surgery, urological surgery, plastic & reconstructive surgery, general surgery, orthopedic surgery, gynecological surgery, thoracic surgery, laparoscopic surgery, arthroscopic surgery.

Why use fat grafts?

Centrifugation creates higher density fat that corresponds to greater cellularity. (8) Greater cellularity corresponds to better results (2, 3) In cosmetic procedures, fat should be injected through smaller gauge needles to in a minimize the distance from the center of the graft to vascular tissue. Generally, minimizing sheer force and injecting in layers between healthy vascularized tissue leads to optimal results. (1,4)
(1) Pu et al “Towards more rationalized approach to autologous fat grafting” Journal of Plastic, Reconstructive & Aesthetic Surgery 2012; 65, 413-419
(2) Allen RJ Jr, “Grading lipoaspirate: is there an optimal density for fat grafting?” Plast Reconstr Surg. 2013 Jan;131(1):38-45.
(3) Condé-Green A, et al; “Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats: a pilot study.” Aesthet Surg J. 2013 Jul;33(5):713-21.
(4) Lee JH, et al;“The effect of pressure and shear on autologous fat grafting.”Plast Reconstr Surg. 2013 May;131(5):1125-36.

Why centrifuge fat?

Centrifugation is a simple method to collect high density fat and remove oil, tumescent fluid and red cells which interfere with the long term engraftment of transplanted fat. When comparing results from transplanted fat based on density gradients, higher density fat generates the best long term results. (1) Lower g-force with a longer spin time is less traumatic on cells. Cells exposed to low g-force have the same viability when compared to cells that have not been centrifuged. (2) This is best captured by the following quote: ”Greater percentages of highest density fractions of lipoaspirate persist over time compared with lowest density fractions. A vasculogenic mechanism appears to contribute significantly, as highest density fractions contain more progenitor cells and increased concentrations of several vasculogenic mediators than lowest density fractions.”(1)
(1) Allen RJ Jr, “Grading lipoaspirate: is there an optimal density for fat grafting?” Plast Reconstr Surg. 2013 Jan;131(1):38-45.
(2) Ferraro GA, et al; “Effects of a new centrifugation method on adipose cell viability for autologous fat grafting.” Aesthetic Plast Surg. 2011 Jun;35(3):341-8.

Why use the syringe method of harvesting fat?

Syringe aspiration is a relatively less traumatic method to harvest fat grafts compared to higher pressure wall suction. (1,2) The syringe technique can be time consuming for the large quantity of fat graft needed for certain procedures and consequently, a need for a means to harvest and process larger volumes of fat is emerging. (3)
(1) Pu, et al “The viability of fatty tissues within adipose aspirates afer conventional liposuction:a comprehensive study. Ann Plast Surg 2005; 54:288-92
(2) Pu, Coleman SR et al “Autologous fat grafts harvested and refined by the coleman technique: a comparative study” Plast Reconstr Surg 2008; 122: 932-7
(3) Pu et al “Towards a more rationalized approach to autologous fat grafting” Journal of Plastic, Reconstructive & Aesthetic Surgery 2012; 65, 413-419

Why do clinicians inject fat into the joint space?

Inside the joint, bleeding occurs after injury, but no fibrin-platelet plug is observed to form.(1) Circulating intra-articular plasmin breaks down the fibrin plug as fast as it can form. (1,3,4) While human Platelets release a fast-acting plasmin inhibitor, the high levels of plasminogen in the synovium overwhelms the natural process of the platelet fibrin clot. (2) Thus healing of tissue inside a joint has proved difficult.(1) Tissue outside the joint such as the medial collateral ligament is typically not exposed to synovial fluid after injury and is not as challenging a tissue to heal. (1) While the cellular composition of the two ligaments are similar, their microenvironments are different. (1) Scaffolds that are not made out of fibrin, such as Collagen, the primary structure of fat, does not degrade in the presence of plasminogen. (1,5) The ideal biologic tissue inside the joint should contain platelets to prevent premature degradation, collagen from fat or other source, growth factors, and stem cells to begin the tissue regeneration process.
1. Martha M. Murray et al Collagen-Platelet Rich Plasma Hydrogel Enhances Primary Repair of the Porcine Anterior Cruciate Ligament J Orthop Res 25:81–91, 2007
2. Hansen et al Partial Purification and Characterization of a New Fast-Acting Plasmin Inhibitor from Human Platelets EVIDENCE FOR NON-IDENTITY WITH THE KNOWN PLASMA PROTEINASE INHIBITORS Biochem. J. (1980) 187, 173-180 7,173-180 173 Printed in Great Britain
3. Rosc D, et al Post-traumatic plasminogenesis in intraarticular exudate in the knee joint. Med. Sci. Monit.2002;8:371–378. [PubMed]
4. Carmassi F, et al Elastase- and plasmin-mediated fibrinolysis in rheumatoid arthritis. Int. J. Tissue React. 1994;16:89–93. [PubMed]
5. Smith SA, et al Polyphosphate enhances fibrin clot structure. Blood.2008;112:2810–2816.

Why use fat verses synthetic filler in cosmetic surgery?

Fat contains high numbers of mesenchymal and CD 34+ stem cells which are anti-inflammatory and have been documented to reduce pain in randomized controlled trials. (1,2)
Improved fat grafting techniques have long lasting results, are less invasive, require less recovery time compared to traditional methods and are consequently gaining in popularity with both surgeons and patients (2,4) In Cosmetic surgery autologous fat grafting uses your own tissue that will age naturally with you so that the youthful appearance is maintained even as you age. The body treats synthetic fillers as foreign objects, which can result in inflammation, infection, migration and granuloma (an inflammatory cyst) formation.
(1) Caviggioli F et al “Autologous fat graft in postmastectomy pain syndrome” Plast Reconstr Surg. 2011 Aug;128(2):349-52.
(2) Manferdini C, et al “Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2.”Arthritis Rheum. 2013 May;65(5):1271-81.
(3) Phulpin B et al “Rehabilitation of irradiated head and neck tissues by autologous fat transplantation.” Plast Reconstr Surg. 2009 Apr;123(4):1187-97.

In cosmetic procedures, why inject fat through a smaller gauge needle and what is the benefit of transferring the aspirated fat from the larger aspiration syringes to smaller injection syringes?

Layering the transplanted fat between vascularized healthy tissue while minimizing sheer force results in the best long term results. Fat that is injected through a smaller gauge needle creates a graft that is smaller in diameter and therefore the distance from the center of the graft to vascularized tissue is minimized. “The studies also reinforce the principle that fat grafts, once placed, should have a maximal amount of contact with the vascularized tissue in the recipient site for better survival.”(1) “Fat grafts injected slowly with low shear stress significantly outperformed fat injected with high shear stress.” (2)
(1) Pu et al “Towards more rationalized approach to autologous fat grafting” Journal of Plastic, Reconstructive & Aesthetic Surgery 2012; 65, 413-419
(2) Lee JH, et al;“The effect of pressure and shear on autologous fat grafting.”Plast Reconstr Surg. 2013 May;131(5):1125-36.