A dramatic decline in the number of HSC and CFU-f is observed with increasing amounts of marrow aspiration. With respect to HSC and in the setting of large volume aspirations (1,000 mL and greater) for allogeneic transplantation, the first 1mL of marrow had 3X (300%) more nucleated cells and 10X (1000%) more stem cells than the overall aspirate. Similarly, it has been documented that the number of MSC per/mL significantly decreases with increased volumes of aspirate taken. The table below demonstrates this phenomena by comparing the number of CFU-f per mL in differing volumes of aspirate in similar patient populations:
Importantly, the number of CFU-f in incremental aspirations of over 100 mL have as little as 60 CFU-f / mL.
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