IS THERE A RELATIONSHIP BETWEEN DIFFERING VOLUMES OF BONE MARROW ASPIRATES AND THEIR CELLULAR COMPOSITION?

A dramatic decline in the number of HSC and CFU-f is observed with increasing amounts of marrow aspiration. With respect to HSC and in the setting of large volume aspirations (1,000 mL and greater) for allogeneic transplantation, the first 1mL of marrow had 3X (300%) more nucleated cells and 10X (1000%) more stem cells than the overall aspirate. Similarly, it has been documented that the number of MSC per/mL significantly decreases with increased volumes of aspirate taken. The table below demonstrates this phenomena by comparing the number of CFU-f per mL in differing volumes of aspirate in similar patient populations:

Volume 1mL 2mL 4mL CFU-f/mL 1458 (5) 675 (4) 254 (6)

Importantly, the number of CFU-f in incremental aspirations of over 100 mL have as little as 60 CFU-f / mL.

Batinic, D et al “Relationship between differing volumes of bone marrow aspirates and their cellular composition.” Bone Marrow Transplant. 1990 Aug;6(2):103-7. 

Jing Li; et al “Factors Affecting Mesenchymal Stromal Cells Yield from Bone Marrow Aspiration” Chin J Cancer Res. Mar 2011; 23(1): 43–48. 

MUSCHLER G, et al “Aspiration to Obtain Osteoblast Progenitor Cells from Human Bone Marrow: The Influence of Aspiration Volume” The Journal of Bone and Joint Surgery; VOL. 79-A, NO. 11, NOVEMBER 1997 

Vishal Hegde MD et al; “Title: A prospective comparison of three approved systems for autologous bone marrow concentration demonstrated non-equivalency in progenitor cell number and concentration.” Journal of Orthopaedic Trauma Publish Ahead of Print